Immunohistochemical Study on the Ruminal Wall of Adult Baladi Goats (Capra hircus)
A total of eight Baladi goats of both sexes (with ages from 11 to 18 months) were used to describe the histological structure of the rumen in addition to investigate the immunohistochemical localization and distribution of ki-67, caspase 3 and vimentin. Histologically, the ruminal wall is consisted of mucosa-submucosa, muscularis and serosa. Ruminal papillae are the characteristics for the ruminal mucosa that were covered by keratinized epithelium. The submucosa is consisted of loose connective tissue lacking glands. Circular and longitudinal smooth muscle layers were the components of muscularis however, the rumen is covered externally by typical serosa. The immunohistochemical study was done using the avidin-biotin immunoperoxidase method. Ki-67 immunostaining was limited to nuclei of basal cells layer in the ruminal epithelium suggesting the importance of ki-67 in epithelial cells proliferation as well as keratin biosynthesis. Caspase 3 immunostaining was localized in cytoplasm and nuclei of some epithelial cells that referred to cell apoptosis. The vimentin immunostaining was widespread in epithelia, fibroblasts in propria and submucosa, and endothelia of blood vessels supports the importance of VIM as an intermediate filament protein. Detection of vimentin in glial cells of enteric plexuses indicates its supportive role in the nervous control of the rumen. Overall, the histological and immunohistochemical findings of this study supports the structure-function relationship of the rumen of Baladi goats.
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